4QIL
Crystal structure of the ROQ domain of human Roquin in complex with the Hmg19 stem-loop RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.600, 170.000, 51.100 |
| Unit cell angles | 90.00, 97.00, 90.00 |
Refinement procedure
| Resolution | 37.696 - 2.900 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qik |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.374 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | COMO |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.094 | 0.415 |
| Number of reflections | 17984 | |
| <I/σ(I)> | 9.1 | 1.7 |
| Completeness [%] | 96.8 | 88.4 |
| Redundancy | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.1 M Tris-HCl, pH 8.2, 0.3 M calcium acetate, 12%-14% PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
