4QBT
Crystal structure of tyrosine bound human tyrosyl tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.251, 162.641, 35.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.630 - 2.100 |
| R-factor | 0.21584 |
| Rwork | 0.213 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q11 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.008 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.630 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.078 | 0.447 |
| Number of reflections | 24513 | |
| <I/σ(I)> | 8.9 | 4.6 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 2.1 M ammonium sulfate, 0.1 M sodium phosphate monobasic, pH 6-8, 2% acetone, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
