4Q9U
Crystal structure of the Rab5, Rabex-5delta and Rabaptin-5C21 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 174.821, 174.821, 148.995 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.781 - 4.618 |
| R-factor | 0.2561 |
| Rwork | 0.251 |
| R-free | 0.34270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ot3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.873 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.760 |
| High resolution limit [Å] | 4.600 | 9.890 | 4.600 |
| Rmerge | 0.117 | 0.048 | 0.943 |
| Number of reflections | 12699 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 97.6 | 98.3 | 95.8 |
| Redundancy | 9.8 | 10.6 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 5 | 289 | 1.0M NaH2PO4/K2HPO4, pH 5.0, hanging drop, temperature 289K |
