4Q9M
Crystal structure of UPPs in complex with FPP and an allosteric inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.850, 91.850, 155.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.850 - 2.060 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.20470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.063 | 0.255 |
| Number of reflections | 41919 | |
| <I/σ(I)> | 41.91 | 6.71 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 11.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
