4Q2G
CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S223C, inactive mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00137 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.410, 141.410, 197.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.100 - 3.400 |
| R-factor | 0.291 |
| Rwork | 0.291 |
| R-free | 0.31500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.100 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 16298 | |
| <I/σ(I)> | 11.1 | 2.9 |
| Completeness [%] | 97.9 | 99.2 |
| Redundancy | 8.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 8%-13% PEG3350, 100mM NaCl, 20mM HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
