4Q2G
CRYSTAL STRUCTURE OF AN INTRAMEMBRANE CDP-DAG SYNTHETASE CENTRAL FOR PHOSPHOLIPID BIOSYNTHESIS (S200C/S223C, inactive mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00137 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 141.410, 141.410, 197.760 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.100 - 3.400 |
R-factor | 0.291 |
Rwork | 0.291 |
R-free | 0.31500 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.700 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.100 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 16298 | |
<I/σ(I)> | 11.1 | 2.9 |
Completeness [%] | 97.9 | 99.2 |
Redundancy | 8.2 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 8%-13% PEG3350, 100mM NaCl, 20mM HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |