4PUQ
Mus Musculus Tdp2 reaction product complex with 5'-phosphorylated RNA/DNA, glycerol, and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.828, 69.258, 167.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.032 - 1.600 |
| R-factor | 0.1309 |
| Rwork | 0.129 |
| R-free | 0.17060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gz1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.209 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX (1.8.2_1309) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
| Rmerge | 0.061 | 0.041 | 0.548 |
| Number of reflections | 83186 | ||
| <I/σ(I)> | 12 | ||
| Completeness [%] | 98.1 | 99.6 | 89.1 |
| Redundancy | 3.5 | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 20% PEG 3350, 0.2M sodium acetate, 5mM MgCl2, pH 7.0, VAPOR DIFFUSION, temperature 277K |
