4PNK

G protein-coupled receptor kinase 2 in complex with GSK180736A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9787
Spacegroup name	C 2 2 21
Unit cell lengths	61.435, 241.398, 212.526
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.550 - 2.560
R-factor	0.19382
Rwork	0.190
R-free	0.25928
RMSD bond length	0.016
RMSD bond angle	1.914
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.630
High resolution limit [Å]	2.560	2.560
Number of reflections	51212
<I/σ(I)>	18.7	1.8
Completeness [%]	100.0	100
Redundancy	26.2	25.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	MES, PEG 3350, NaCl