4OCT
Crystal structure of human ALKBH5 crystallized in the presence of Mn^{2+} and 2-oxoglutarate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-05-23 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.930, 57.234, 78.407 |
Unit cell angles | 90.00, 101.07, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.280 |
R-factor | 0.2405 |
Rwork | 0.238 |
R-free | 0.28410 |
Structure solution method | direct placement |
Starting model (for MR) | related to pdb entry 4O61 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.401 |
Data reduction software | XDS |
Data scaling software | Aimless (0.2.14) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.470 | 38.470 | 2.360 |
High resolution limit [Å] | 2.280 | 8.830 | 2.280 |
Rmerge | 0.127 | 0.011 | 0.011 |
Total number of observations | 2299 | 13193 | |
Number of reflections | 18808 | ||
<I/σ(I)> | 13.3 | 52.5 | 2.1 |
Completeness [%] | 99.9 | 98.9 | 100 |
Redundancy | 7.3 | 6.6 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 0.005 M of each manganous sulfate and 2-oxyglutarate were added to the protein stock solution. Reservoir: 20% PEG-3350, 0.2 M diammonium phosphate, vapor diffusion, temperature 291K |