4O6Z
Crystal structure of serine hydroxymethyltransferase with covalently bound PLP Schiff-base from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 |
| Unit cell lengths | 254.947, 254.947, 61.399 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.850 - 2.980 |
| R-factor | 0.22831 |
| Rwork | 0.224 |
| R-free | 0.26959 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.536 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.090 |
| High resolution limit [Å] | 2.980 | 6.400 | 2.980 |
| Rmerge | 0.052 | 0.032 | 0.206 |
| Number of reflections | 47100 | ||
| <I/σ(I)> | 32.6 | 31.79 | 10.16 |
| Completeness [%] | 99.8 | 99.9 | 99.2 |
| Redundancy | 8 | 7.6 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 293 | MES, PEG 4000, MgCl2, pH 6.5, Microbatch, temperature 293K |
