4NN6

Cytokine receptor complex - Crystal form 1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.968600
Spacegroup name	P 21 21 2
Unit cell lengths	147.949, 75.157, 51.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.449 - 2.545
R-factor	0.2606
Rwork	0.259
R-free	0.29660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nn5
RMSD bond length	0.003
RMSD bond angle	0.860
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.545	2.545
Rmerge	0.108	0.846
Number of reflections	19440
<I/σ(I)>	11.2	1.87
Completeness [%]	99.5	97.8
Redundancy	6.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.1	293	14% PEG4000, 0.3 M sodium acetate, pH 4.5, 0.3 M calcium acetate, soaked in 18% PEG4000, 0.1 M Tris-HCl, pH 7.5, 0.3 M calcium acetate for 1 hour prior to cryoprotection, VAPOR DIFFUSION, SITTING DROP, temperature 293K