4NDF
Human Aprataxin (Aptx) bound to RNA-DNA, AMP, and Zn - product complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.429, 116.211, 117.141 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.409 - 1.944 |
R-factor | 0.1729 |
Rwork | 0.171 |
R-free | 0.20470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ndh |
RMSD bond length | 0.009 |
RMSD bond angle | 1.154 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
High resolution limit [Å] | 1.944 | 4.200 | 1.944 |
Rmerge | 0.078 | 0.033 | 0.588 |
Number of reflections | 41518 | ||
<I/σ(I)> | 16.8 | ||
Completeness [%] | 99.9 | 99.6 | 99.9 |
Redundancy | 4 | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | 200 mM potassium sodium tartrate, 20% w/v PEG3350, pH 8.5, VAPOR DIFFUSION, temperature 277K |