4MVC
Crystal Structure of a Mammalian Cytidylyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-08 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.260, 44.550, 97.130 |
| Unit cell angles | 90.00, 111.21, 90.00 |
Refinement procedure
| Resolution | 39.974 - 3.000 |
| R-factor | 0.2262 |
| Rwork | 0.223 |
| R-free | 0.28780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hl4 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.958 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.141 | 0.339 |
| Number of reflections | 13085 | |
| <I/σ(I)> | 7 | 3.3 |
| Completeness [%] | 92.2 | 92.3 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | 0.1 M Tris (pH 8.5), 20% PEG 10,000, 0.2 M sodium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
