4MQW

Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor (P31)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	110
Detector technology	CCD
Collection date	2011-05-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 31
Unit cell lengths	95.900, 95.900, 204.281
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.930 - 2.900
R-factor	0.1769
Rwork	0.174
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ay9
RMSD bond length	0.009
RMSD bond angle	1.210
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASES
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	3.270	2.950
High resolution limit [Å]	2.900	3.190	2.900
Rmerge	0.079
Number of reflections	43220
<I/σ(I)>	19.9
Completeness [%]	95.5	97.2	70

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	The FSH-FSHRED complex was concentrated by ultrafiltration to about 10 mg/ml in HBS. 0.1M imidazole and 20% Jeffamine M-600, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K