4MQW
Structure of follicle-stimulating hormone in complex with the entire ectodomain of its receptor (P31)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-05-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 |
Unit cell lengths | 95.900, 95.900, 204.281 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.930 - 2.900 |
R-factor | 0.1769 |
Rwork | 0.174 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ay9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.210 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.270 | 2.950 |
High resolution limit [Å] | 2.900 | 3.190 | 2.900 |
Rmerge | 0.079 | ||
Number of reflections | 43220 | ||
<I/σ(I)> | 19.9 | ||
Completeness [%] | 95.5 | 97.2 | 70 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | The FSH-FSHRED complex was concentrated by ultrafiltration to about 10 mg/ml in HBS. 0.1M imidazole and 20% Jeffamine M-600, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |