4LZ2

Crystal structure of the bromodomain of human BAZ2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 31 2 1
Unit cell lengths	95.417, 95.417, 33.054
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.560 - 1.760
R-factor	0.178
Rwork	0.176
R-free	0.21719
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3GOL 1dvv 1x0j 3dai 3hmh 2grc 2oo1 2oss 2UOU 3d7c AND 3DWY
RMSD bond length	0.015
RMSD bond angle	1.624
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.560	27.540	1.860
High resolution limit [Å]	1.760	5.570	1.760
Rmerge	0.063	0.032	0.850
Number of reflections	17392
<I/σ(I)>	20	57.1	2.6
Completeness [%]	100.0	99.3	100
Redundancy	9.9	9.1	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.4	277	22% PEG3350, 0.1 M Tris, pH 8.4, 0.25 M magnesium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 277K