4LL1
The structure of the TRX and TXNIP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 173 |
Detector technology | CCD |
Collection date | 2009-07-22 |
Wavelength(s) | 1.100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.135, 64.022, 88.295 |
Unit cell angles | 90.00, 91.28, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.22399 |
Rwork | 0.220 |
R-free | 0.28806 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Partially refined SeMet-substituted TRX-TXNIP complex structure |
RMSD bond length | 0.015 |
RMSD bond angle | 1.594 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 58935 | |
Completeness [%] | 96.6 | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294.15 | 0.16M tri-sodium citrate, 16% PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.15K |