4LGY
Importance of Hydrophobic Cavities in Allosteric Regulation of Formylglycinamide Synthetase: Insight from Xenon Trapping and Statistical Coupling Analysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Detector technology | CCD |
Collection date | 2013-02-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97372 |
Spacegroup name | P 65 |
Unit cell lengths | 146.329, 146.329, 140.841 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.500 - 1.480 |
R-factor | 0.14204 |
Rwork | 0.142 |
R-free | 0.16623 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t3t |
RMSD bond length | 0.031 |
RMSD bond angle | 2.855 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.500 | |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.067 | |
Number of reflections | 274143 | |
<I/σ(I)> | 11.3 | |
Completeness [%] | 96.5 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 2M ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP |