4LGD
Structural Basis for Autoactivation of Human Mst2 Kinase and Its Regulation by RASSF5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-12 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.403, 237.136, 95.862 |
Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.050 |
R-factor | 0.202 |
Rwork | 0.199 |
R-free | 0.24400 |
Structure solution method | MR-SAD |
Starting model (for MR) | 4lg4 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.610 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.049 | 3.100 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.109 | 0.762 |
Number of reflections | 50456 | |
<I/σ(I)> | 14.4 | 1.6 |
Completeness [%] | 98.2 | 90.7 |
Redundancy | 4.1 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1 M Bis-Tris propane, 200 mM Na2SO4, 20% (w/v) PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |
2 |