4LGD

Structural Basis for Autoactivation of Human Mst2 Kinase and Its Regulation by RASSF5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Serine/threonine-protein kinase 3	polymer	378	43626.2	4	UniProt (Q13188) Pfam (PF00069) Pfam (PF11629) In PDB	Homo sapiens (human)	Mammalian STE20-like protein kinase 2, MST-2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1, Serine/threonine-protein kinase 3 36kDa subunit, MST2/N, Serine/threonine-protein kinase 3 20kDa subunit, MST2/C
2	E, F, G, H	Ras association domain family member 5, RASSF5	polymer	49	5963.7	4	UniProt (Q8WWW0) Pfam (PF16517) In PDB	Homo sapiens (human)	New ras effector 1, Regulator for cell adhesion and polarization enriched in lymphoid tissues, RAPL
3	A, B, C, D	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	4	Chemie (ANP)
4	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	D, A, B, C	SULFATE ION	non-polymer		96.1	9	Chemie (SO4)
6	D, C	SODIUM ION	non-polymer		23.0	3	Chemie (NA)

Sequence modifications

A, B, C, D: 9 - 313 (UniProt: Q13188)

PDB	External Database	Details
Met 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
Gly 7	-	expression tag
Ser 8	-	expression tag
Asn 146	Asp 146	engineered mutation

A, B, C, D: 428 - 491 (UniProt: Q13188)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	198359.3
Non-Polymers*	Number of molecules	20
	Total formula weight	3055.5
All*	Total formula weight	201414.8

*Water molecules are not included.