4LG0

Structure of a ternary FOXO1-ETS1 DNA complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075,0.9793,0.979
Spacegroup name	C 2 2 21
Unit cell lengths	68.658, 114.944, 136.325
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.938 - 2.190
R-factor	0.1968
Rwork	0.194
R-free	0.24300
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.584
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.8.0047)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.280
High resolution limit [Å]	2.190	2.200
Rmerge	0.111
Number of reflections	27722
<I/σ(I)>	24.1
Completeness [%]	99.5	95.4
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	30% PEG 400, 0.05M 2-Amino-2-hydroxymethyl-propane-1,3-diol, 0.1M potassium chloride, 0.01M magnesium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K