4LC2
Crystal structure of the bromodomain of human BRPF1B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-06-14 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.835, 60.835, 63.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.000 - 1.650 |
R-factor | 0.1767 |
Rwork | 0.174 |
R-free | 0.21980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ensemble of PDB ENTRIES 1DVV 1x0j 3dai 3hmh 2grc 2oo1 2oss 2ouo 3d7c 3dwy |
RMSD bond length | 0.016 |
RMSD bond angle | 1.615 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.000 | 19.000 | 1.740 |
High resolution limit [Å] | 1.650 | 5.220 | 1.650 |
Rmerge | 0.026 | 0.019 | 0.135 |
Number of reflections | 16793 | ||
<I/σ(I)> | 40.1 | 21.7 | 5.8 |
Completeness [%] | 99.7 | 97.6 | 98.3 |
Redundancy | 6.7 | 6.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 277 | 20% PEG3350 0.1 M bis tris propane, 10% ethylene glycol 0.15 M sodium nitrate, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 277K |