4KYO

Alanine-glyoxylate aminotransferase variant K390A in complex with the TPR domain of human Pex5p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-22
Detector	MAR CCD 165 mm
Wavelength(s)	0.812
Spacegroup name	P 1
Unit cell lengths	57.556, 74.830, 91.751
Unit cell angles	87.23, 83.41, 89.83

Refinement procedure

Resolution	19.740 - 2.200
R-factor	0.17738
Rwork	0.175
R-free	0.21753
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3r9a
RMSD bond length	0.008
RMSD bond angle	1.245
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	51.030
High resolution limit [Å]	2.200
Rmerge	0.114
Number of reflections	73170
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	0.1 M Bis-Tris (pH=5.3), 0.12 M LiSO4, 17% (w/w) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K