4KAZ
Crystal structure of RNase T in complex with a Y structured DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-03 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 60.675, 90.580, 46.621 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.767 - 1.900 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ngy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.148 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 20966 | |
<I/σ(I)> | 32.23 | 3.92 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.5 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% v/v 2-Propanol, 0.1 M MES monohydrate, 20% w/v Polyethylene glycol monomethyl ester 2000 , pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |