4KAZ

Crystal structure of RNase T in complex with a Y structured DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease T	polymer	235	25719.0	1	UniProt (P30014) Pfam (PF00929)	Escherichia coli	Exoribonuclease T, RNase T
2	B (B)	DNA (5'-D(*TP*TP*GP*GP*CP*CP*CP*TP*CP*TP*TP*TP*AP*GP*GP*GP*CP*CP*CP*C)-3')	polymer	20	6052.9	1
3	C, D (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E, F (A, B)	water	water		18.0	168	Chemie (HOH)

Sequence modifications

A: 1 - 215 (UniProt: P30014)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31771.9
Non-Polymers*	Number of molecules	2
	Total formula weight	48.6
All*	Total formula weight	31820.5

*Water molecules are not included.