3NGY
Crystal structure of RNase T (E92G mutant)
Summary for 3NGY
| Entry DOI | 10.2210/pdb3ngy/pdb |
| Related | 3NGZ 3NH0 3NH1 3NH2 |
| Descriptor | Ribonuclease T, his tag sequence, COBALT (II) ION, ... (4 entities in total) |
| Functional Keywords | exoribonuclease, rna processing, rna maturation, protein-dna interactions, exo-nuclease, hydrolase |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 5 |
| Total formula weight | 103560.52 |
| Authors | Hsiao, Y.-Y.,Yuan, H.S. (deposition date: 2010-06-14, release date: 2011-02-16, Last modification date: 2024-11-06) |
| Primary citation | Hsiao, Y.-Y.,Yang, C.-C.,Lin, C.L.,Lin, J.L.J.,Duh, Y.,Yuan, H.S. Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation Nat.Chem.Biol., 7:236-243, 2011 Cited by PubMed Abstract: RNA maturation relies on various exonucleases to remove nucleotides successively from the 5' or 3' end of nucleic acids. However, little is known regarding the molecular basis for substrate and cleavage preference of exonucleases. Our biochemical and structural analyses on RNase T-DNA complexes show that the RNase T dimer has an ideal architecture for binding a duplex with a short 3' overhang to produce a digestion product of a duplex with a 2-nucleotide (nt) or 1-nt 3' overhang, depending on the composition of the last base pair in the duplex. A 'C-filter' in RNase T screens out the nucleic acids with 3'-terminal cytosines for hydrolysis by inducing a disruptive conformational change at the active site. Our results reveal the general principles and the working mechanism for the final trimming step made by RNase T in the maturation of stable RNA and pave the way for the understanding of other DEDD family exonucleases. PubMed: 21317904DOI: 10.1038/nchembio.524 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.204 Å) |
Structure validation
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