3NGY

Crystal structure of RNase T (E92G mutant)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ribonuclease T	polymer	235	25647.0	4	UniProt (P30014) Pfam (PF00929)	Escherichia coli	RNase T, Exoribonuclease T
2	E (E)	his tag sequence	polymer	6	795.8	1		Escherichia coli
3	F, G, H (A, B, D)	COBALT (II) ION	non-polymer		58.9	3	Chemie (CO)
4	I, J, K, L, M (A, B, C, D, E)	water	water		18.0	373	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 215 (UniProt: P30014)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 92	Glu 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	103383.7
Non-Polymers*	Number of molecules	3
	Total formula weight	176.8
All*	Total formula weight	103560.5

*Water molecules are not included.