4JUO
A low-resolution three-gate structure of topoisomerase IV from Streptococcus pneumoniae in space group H32
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97630 |
Spacegroup name | H 3 2 |
Unit cell lengths | 213.580, 213.580, 211.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.442 - 6.530 |
R-factor | 0.2498 |
Rwork | 0.247 |
R-free | 0.29820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rae |
RMSD bond length | 0.017 |
RMSD bond angle | 1.491 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.400 | 6.710 |
High resolution limit [Å] | 6.530 | 6.530 |
Rmerge | 0.060 | 0.410 |
Number of reflections | 3701 | |
<I/σ(I)> | 6.84 | 1.88 |
Completeness [%] | 99.2 | 99.66 |
Redundancy | 9.66 | 10.93 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 3-4% isopropanol, 50 mM Na cacodylate, optimised mixture of salts, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |