4JUO

A low-resolution three-gate structure of topoisomerase IV from Streptococcus pneumoniae in space group H32

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA topoisomerase 4 subunit A	polymer	496	56455.4	1	UniProt (P72525) Pfam (PF00521) In PDB	Streptococcus pneumoniae	Topoisomerase IV subunit A
2	C	DNA topoisomerase 4 subunit B	polymer	670	74528.2	1	UniProt (Q59961) Pfam (PF02518) Pfam (PF00204) Pfam (PF01751) Pfam (PF00986) In PDB	Streptococcus pneumoniae serotype 4
3	E	E-site DNA	polymer	11	3397.2	1
4	F	E-site DNA	polymer	15	4544.0	1
5	G	E-site DNA	polymer	11	3339.2	1
6	H	E-site DNA	polymer	15	4602.0	1
7	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	F	(3S)-9-fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid	non-polymer		361.4	1	Chemie (LFX)

Sequence modifications

A: 1 - 488 (UniProt: P72525)

PDB	External Database	Details
Thr 257	Ile 257	conflict
Leu 489	-	expression tag
Glu 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag
His 494	-	expression tag
His 495	-	expression tag
His 496	-	expression tag

C: 1 - 647 (UniProt: Q59961)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag
Asp 217	Asn 217	conflict
Ile 460	Val 460	conflict
Ala 644	Thr 644	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	146866.1
Non-Polymers*	Number of molecules	3
	Total formula weight	410.0
All*	Total formula weight	147276.0

*Water molecules are not included.