4IZK
Crystal structure of yellowtail ascites virus VP4 protease active site mutant (K674A) reveals both an acyl-enzyme complex and an empty active site
Replaces: 4HHCReplaces: 3RBPExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9795 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 273.520, 273.520, 273.520 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.800 - 2.300 |
R-factor | 0.17394 |
Rwork | 0.173 |
R-free | 0.19685 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4izj |
RMSD bond length | 0.012 |
RMSD bond angle | 1.428 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.800 | 2.400 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.105 | 0.249 |
Number of reflections | 39388 | |
<I/σ(I)> | 13.8 | 6.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.7 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 296 | 25% PEG 2000, 0.1 M MES, 0.45 M MAGNESIUM CHLORIDE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |