4IZK

Crystal structure of yellowtail ascites virus VP4 protease active site mutant (K674A) reveals both an acyl-enzyme complex and an empty active site

Replaces:  4HHCReplaces:  3RBP

Summary for 4IZK

• Entry DOI: 10.2210/pdb4izk/pdb
• Related: 2GEF 2PNL 2PNM 3P06 4IZJ
• Descriptor: Yellowtail Ascites Virus (YAV) VP4 protease, MAGNESIUM ION, BETA-MERCAPTOETHANOL, ... (5 entities in total)
• Functional Keywords: viral protease, birnavirus, serine-lysine dyad mechanism, alpha-beta protein fold, lysine general base, acyl-enzyme, product complex, hydrolase
• Biological source: Yellowtail ascites virus; More
• Cellular location: Host cytoplasm . Virion : P89521 P89521
• Total number of polymer chains: 2
• Total formula weight: 44649.41

Authors

Paetzel, M.,Chung, I.Y.W. (deposition date: 2013-01-30, release date: 2013-02-27, Last modification date: 2023-12-06)

• Primary citation: Chung, I.Y.,Paetzel, M.; Crystal Structures of Yellowtail Ascites Virus VP4 Protease: TRAPPING AN INTERNAL CLEAVAGE SITE TRANS ACYL-ENZYME COMPLEX IN A NATIVE SER/LYS DYAD ACTIVE SITE.; J.Biol.Chem., 288:13068-13081, 2013; PubMed: 23511637; DOI: 10.1074/jbc.M112.386953

Experimental method

X-RAY DIFFRACTION (2.3 Å)