4IOX
The structure of the herpes simplex virus DNA-packaging motor pUL15 C-terminal nuclease domain provides insights into cleavage of concatemeric viral genome precursors
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 96.944, 96.944, 194.034 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.799 - 2.458 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.24680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n4p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.350 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 | 2.500 |
High resolution limit [Å] | 2.458 | 3.100 | 2.458 |
Rmerge | 0.086 | 0.140 | 0.480 |
Number of reflections | 34365 | ||
<I/σ(I)> | 42.92 | 21.6 | 2.6 |
Completeness [%] | 99.6 | 100 | 100 |
Redundancy | 12.2 | 12.1 | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1 M ammonium citrate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |