4IJE

Crystal structure of the Zaire ebolavirus VP35 interferon inhibitory domain R312A/K319A/R322A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792075
Spacegroup name	P 61 2 2
Unit cell lengths	81.012, 81.012, 344.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.096 - 1.899
R-factor	0.195
Rwork	0.193
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fke
RMSD bond length	0.019
RMSD bond angle	0.880
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.899	5.160	1.899
Rmerge	0.130	0.058
Number of reflections	54187
<I/σ(I)>	9.3
Completeness [%]	99.8	98.8	99.5
Redundancy	9.1	9.3	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298.15	2.2 M sodium potassium phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K