4IHV
Crystal structure of Fis bound to 27 bp sequence DNA F28 (AAATTTGTTTGAGCGTTGAGCAAATTT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.92017 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.790, 89.390, 154.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.927 - 2.716 |
R-factor | 0.221 |
Rwork | 0.217 |
R-free | 0.25750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iv5 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.491 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.1) |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.790 | |
High resolution limit [Å] | 2.716 | 12.150 | 2.716 |
Rmerge | 0.082 | 0.034 | 0.880 |
Number of reflections | 16603 | 219 | 1173 |
<I/σ(I)> | 16.06 | 31.91 | 2.38 |
Completeness [%] | 99.6 | 94 | 97.5 |
Redundancy | 6.99 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M sodium citrate, 0.1 M Tris-HCl, pH 8.5, 36% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 277K |