4IG8

Structural basis for cytosolic double-stranded RNA surveillance by human OAS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	77
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	71.200, 93.200, 98.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.700 - 2.700
R-factor	0.2311
Rwork	0.229
R-free	0.28270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.776
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	67.700
High resolution limit [Å]	2.300
Number of reflections	18604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	293	10 mM HEPES (pH 7.4), 350 mM NaCl, and 5 mM MgCl2, VAPOR DIFFUSION, temperature 293K