4I1O
Crystal structure of the Legionella pneumophila GAP domain of LepB in complex with Rab1b bound to GDP and BeF3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 31 |
Unit cell lengths | 124.410, 124.410, 147.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.157 - 2.701 |
R-factor | 0.2191 |
Rwork | 0.217 |
R-free | 0.24860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 3NKV AND 4I1M |
RMSD bond length | 0.014 |
RMSD bond angle | 1.057 |
Data scaling software | XSCALE |
Phasing software | PHASER (MR) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.157 | 49.157 | 2.800 |
High resolution limit [Å] | 2.700 | 20.000 | 2.700 |
Rmerge | 0.094 | 0.041 | 0.717 |
Number of reflections | 70034 | ||
<I/σ(I)> | 19.47 | 58.06 | 3.67 |
Completeness [%] | 100.0 | 100 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 0.1 M sodium cacodylate, pH 6.4, 0.2 M magnesium chloride, 17% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |