4HOO

Crystal structure of human JMJD2D/KDM4D apoenzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Detector technology	CCD
Collection date	2010-10-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9786
Spacegroup name	P 32
Unit cell lengths	73.119, 73.119, 136.028
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.930 - 2.495
R-factor	0.2092
Rwork	0.207
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hon
RMSD bond length	0.013
RMSD bond angle	1.495
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.000	18.000	2.570
High resolution limit [Å]	2.495	5.670	2.495
Rmerge	0.078	0.037	0.514
Number of reflections	25850
<I/σ(I)>	15.5
Completeness [%]	91.5	100	87
Redundancy	5.3	6.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	0.2M calcium acetate, 0.1M HEPES pH 7.5, 10% PEG 8000, vapor diffusion, hanging drop, temperature 277.15K