4HJJ

Structure Reveals Function of the Dual Variable Domain Immunoglobulin (DVD-Ig) Molecule

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	274.385, 65.498, 78.802
Unit cell angles	90.00, 95.20, 90.00

Refinement procedure

Resolution	21.170 - 2.100
R-factor	0.1856
Rwork	0.184
R-free	0.21950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	136.628	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.049
Number of reflections	80760
Completeness [%]	99.2	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	2M Ammonium Sulfate, 0.1M Sodium Acetate., pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K