4H9G
Probing EF-Tu with a very small brominated fragment library identifies the CCA pocket
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.205, 98.627, 39.751 |
| Unit cell angles | 90.00, 96.06, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.930 |
| R-factor | 0.1558 |
| Rwork | 0.154 |
| R-free | 0.18420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.157 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 1.960 |
| High resolution limit [Å] | 1.852 | 1.850 |
| Rmerge | 0.196 | 0.139 |
| <I/σ(I)> | 8.6 | 1.17 |
| Completeness [%] | 91.3 | |
| Redundancy | 2.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 292 | 1.8M ammonium sulfate, 15% sucrose, 0.1M tris, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
