4H8J

Structure of GluA2-LBD in complex with MES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-28
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	61.987, 92.020, 196.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 1.800
R-factor	0.1755
Rwork	0.173
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bfu
RMSD bond length	0.020
RMSD bond angle	1.733
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	1.900
High resolution limit [Å]	1.800	10.000	1.800
Rmerge	0.105	0.031	0.521
Number of reflections	104825	615	15487
<I/σ(I)>	15.23	36.93	3.89
Completeness [%]	99.9	85.4	100
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	23-27% w/v PEG3350, 0.2 M lithium sulfate, MES sodium, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K