4H3Z
Crystal structure of a symmetric dimer of a tRNA (guanine-(N(1)-)-methyltransferase from Burkholderia phymatum bound to S-adenosyl homocystein in both half-sites
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-28 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 58.850, 188.090, 127.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.200 - 2.150 |
R-factor | 0.1886 |
Rwork | 0.186 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4h3y |
RMSD bond length | 0.011 |
RMSD bond angle | 1.467 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.210 | |
High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
Rmerge | 0.073 | 0.023 | 0.484 |
Number of reflections | 38958 | 494 | 2845 |
<I/σ(I)> | 18.35 | 59.31 | 4.7 |
Completeness [%] | 99.8 | 97.6 | 99.8 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | BuphA.00054.a.A1 PS01368 at 35 mg/mL with 3 mM SAH against CSHT screen condition E10, 0.2 M NaCl, 0.1 M NaOAc pH 4.6, 30% MPD, crystal tracking ID 236894e10, unique puck ID zeh2-4, VAPOR DIFFUSION, SITTING DROP, temperature 289K |