4H3Z

Crystal structure of a symmetric dimer of a tRNA (guanine-(N(1)-)-methyltransferase from Burkholderia phymatum bound to S-adenosyl homocystein in both half-sites

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	tRNA (guanine-N(1)-)-methyltransferase	polymer	276	30788.9	2	UniProt (B2JF31) Pfam (PF01746) In PDB	Burkholderia phymatum	M1G-methyltransferase, tRNA [GM37] methyltransferase
2	A, B	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
3	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4		water	water		18.0	223	Chemie (HOH)

Sequence modifications

A, B: 1 - 255 (UniProt: B2JF31)

PDB	External Database	Details
Met -20	-	expression tag
Ala -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Met -12	-	expression tag
Gly -11	-	expression tag
Thr -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Ala -7	-	expression tag
Gln -6	-	expression tag
Thr -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	61577.9
Non-Polymers*	Number of molecules	4
	Total formula weight	839.7
All*	Total formula weight	62417.6

*Water molecules are not included.