4H34
Crystal structure of the tyrosine phosphatase SHP-2 with Q506P mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 54.790, 202.350, 44.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.120 - 2.700 |
R-factor | 0.2272 |
Rwork | 0.226 |
R-free | 0.24190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2shp |
RMSD bond length | 0.007 |
RMSD bond angle | 0.910 |
Data reduction software | XDS |
Data scaling software | XSCALL |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.139 | 0.559 |
Number of reflections | 14342 | |
<I/σ(I)> | 12.05 | 2.81 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.45 | 6.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 0.02M DDT, 0.1M Tris pH8.0, 19% PEG4000, 10% Glycerol, VAPOR DIFFUSION, temperature 293K |