4GRW
Structure of a complex of human IL-23 with 3 Nanobodies (Llama vHHs)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-07 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.270, 135.140, 139.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.830 - 2.550 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d85 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.610 |
| High resolution limit [Å] | 2.540 | 2.550 |
| Rmerge | 0.082 | 0.601 |
| Number of reflections | 63755 | |
| <I/σ(I)> | 13.5 | 2.65 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 277 | 16.5 % PEG 20K and 0.1M MES, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
