4GNK
Crystal structure of Galphaq in complex with full-length human PLCbeta3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-03-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.13 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.423, 188.845, 293.857 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 4.000 |
R-factor | 0.21588 |
Rwork | 0.214 |
R-free | 0.25468 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3OHM |
RMSD bond length | 0.005 |
RMSD bond angle | 0.858 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 4.070 |
High resolution limit [Å] | 4.000 | 4.000 |
Rmerge | 0.140 | 0.329 |
Number of reflections | 34695 | |
<I/σ(I)> | 8.8 | 1.9 |
Completeness [%] | 76.7 | 36.7 |
Redundancy | 3.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 277.15 | 100 mM MES pH 6.75, 100-200 mM NaCl, and 11-12% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 277.15 | 100 mM MES pH 6.75, 100-200 mM NaCl, and 11-12% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |