4GMJ
Structure of human NOT1 MIF4G domain co-crystallized with CAF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-14 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.470, 101.740, 142.200 |
Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
Resolution | 76.700 - 2.700 |
R-factor | 0.2153 |
Rwork | 0.214 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d5r |
RMSD bond length | 0.006 |
RMSD bond angle | 0.806 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.210 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.112 | 0.676 |
Number of reflections | 63214 | |
<I/σ(I)> | 11 | 2.5 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | 0.1M Tris-HCl, 16% PEG8000, 0.2M MgCl2, 0.2M AMSO4, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |