4GLY
Human urokinase-type plasminogen activator uPA in complex with the two-disulfide bridge peptide UK504
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-06-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 120.800, 120.800, 43.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.130 - 1.518 |
R-factor | 0.1318 |
Rwork | 0.128 |
R-free | 0.20416 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nwn |
RMSD bond length | 0.020 |
RMSD bond angle | 1.996 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | REFMAC (5) |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.130 | 1.600 |
High resolution limit [Å] | 1.518 | 1.518 |
Rmerge | 0.099 | 0.318 |
Number of reflections | 36222 | |
<I/σ(I)> | 3.8 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.6 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 1.8M Ammonium sulfate, 5% PEG400, 0.05% Sodium azide, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |