4GL9

Crystal structure of inhibitory protein SOCS3 in complex with JAK2 kinase domain and fragment of GP130 intracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95371
Spacegroup name	P 65
Unit cell lengths	139.262, 139.262, 316.742
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.580 - 3.900
R-factor	0.254
Rwork	0.249
R-free	0.28080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.608
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.584
High resolution limit [Å]	3.900
Number of reflections	31617
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	0.33M di-potassium hydrogen phosphate, 1.67M sodium dihydrogen phosphate, 0.1M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K