4GKO
Crystal structure of the calcium2+-bound human IgE-Fc(epsilon)3-4 bound to its B cell receptor derCD23
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-09-24 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9173 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.830, 110.130, 367.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.500 - 3.300 |
R-factor | 0.2745 |
Rwork | 0.273 |
R-free | 0.31180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ezm |
RMSD bond length | 0.020 |
RMSD bond angle | 1.963 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.500 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 33757 | |
Completeness [%] | 86.3 | 86.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M sodium acetate trihydrate, 0.1 M Tris pH 8.5, 16% PEG 4000, 10 mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 291K |