4GCW
Crystal structure of RNase Z in complex with precursor tRNA(Thr)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9800 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 174.080, 42.550, 110.890 |
Unit cell angles | 90.00, 127.18, 90.00 |
Refinement procedure
Resolution | 43.300 - 3.000 |
R-factor | 0.2284 |
Rwork | 0.227 |
R-free | 0.25640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fk6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.070 |
Data reduction software | XDS (PACKAGE) |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.310 | 3.160 |
High resolution limit [Å] | 2.980 | 2.980 |
Number of reflections | 13408 | |
<I/σ(I)> | 10.71 | 2.06 |
Completeness [%] | 97.9 | 94.6 |
Redundancy | 6.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 28% PEG MME 2K, 0.1 M Na-MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |