2FK6

Crystal Structure of RNAse Z/tRNA(Thr) complex

Summary for 2FK6

• Entry DOI: 10.2210/pdb2fk6/pdb
• Related: 1WW1 1Y44
• Descriptor: TRNA(THR), RIBONUCLEASE Z, ZINC ION, ... (6 entities in total)
• Functional Keywords: protein-trna complex; zinc-dependent metal hydrolase; trna maturase, hydrolase-rna complex, hydrolase/rna
• Biological source: Bacillus subtilis; More
• Total number of polymer chains: 2
• Total formula weight: 61440.45

Authors

Li de la Sierra-Gallay, I.,Mathy, N.,Pellegrini, O.,Condon, C. (deposition date: 2006-01-04, release date: 2006-03-21, Last modification date: 2023-08-30)

Primary citation

Li de la Sierra-Gallay, I.,Mathy, N.,Pellegrini, O.,Condon, C.
Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA.
Nat.Struct.Mol.Biol., 13:376-377, 2006

PubMed Abstract: The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic removal of the 3' extension of the majority of tRNA precursors. Here we present the structure of the complex between Bacillus subtilis RNase Z and tRNA(Thr), the first structure of a ribonucleolytic processing enzyme bound to tRNA. Binding of tRNA to RNase Z causes conformational changes in both partners to promote reorganization of the catalytic site and tRNA cleavage.

PubMed: 16518398
DOI: 10.1038/nsmb1066

Experimental method

X-RAY DIFFRACTION (2.9 Å)