4GCW
Crystal structure of RNase Z in complex with precursor tRNA(Thr)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ribonuclease Z | polymer | 320 | 35632.6 | 1 | UniProt (P54548) Pfam (PF23023) Pfam (PF12706) | Bacillus subtilis subsp. subtilis | RNase Z, tRNA 3 endonuclease, tRNase Z |
| 2 | B (B) | TRNA(THR) | polymer | 76 | 24597.7 | 1 | |||
| 3 | C, D (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 4 | E (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 5 | F, G (A, B) | water | water | 18.0 | 32 | Chemie (HOH) |
Sequence modifications
A: 1 - 307 (UniProt: P54548)
| PDB | External Database | Details |
|---|---|---|
| Met 46 | Ile 46 | engineered mutation |
| Met 228 | Leu 228 | engineered mutation |
| Lys 308 | - | expression tag |
| Leu 309 | - | expression tag |
| Ala 310 | - | expression tag |
| Ala 311 | - | expression tag |
| Ala 312 | - | expression tag |
| Leu 313 | - | expression tag |
| Glu 314 | - | expression tag |
| His 315 | - | expression tag |
| His 316 | - | expression tag |
| His 317 | - | expression tag |
| His 318 | - | expression tag |
| His 319 | - | expression tag |
| His 320 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 60230.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 326.1 | |
| All* | Total formula weight | 60556.4 |
