4GCW

Crystal structure of RNase Z in complex with precursor tRNA(Thr)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ribonuclease Z	polymer	320	35632.6	1	UniProt (P54548) Pfam (PF12706) In PDB	Bacillus subtilis subsp. subtilis	RNase Z, tRNA 3 endonuclease, tRNase Z
2	B	TRNA(THR)	polymer	76	24597.7	1
3	A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
5		water	water		18.0	32	Chemie (HOH)

Sequence modifications

A: 1 - 307 (UniProt: P54548)

PDB	External Database	Details
Met 46	Ile 46	engineered mutation
Met 228	Leu 228	engineered mutation
Lys 308	-	expression tag
Leu 309	-	expression tag
Ala 310	-	expression tag
Ala 311	-	expression tag
Ala 312	-	expression tag
Leu 313	-	expression tag
Glu 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60230.3
Non-Polymers*	Number of molecules	3
	Total formula weight	326.1
All*	Total formula weight	60556.4

*Water molecules are not included.